Journal: The Journal of Biological Chemistry

Article Title: Organ-specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice *

doi: 10.1074/jbc.M111.290262

Figure Lengend Snippet: Immunohistochemistry of HS in adult kidneys. Cryostat sections of the adult kidneys from wild-type (A and D), Sulf1−/− (B and E), and Sulf2−/− (C and F) mice were incubated with anti-HS antibodies RB4CD12 (A–C) or AO4B08 (D–F). The antibody binding was detected by incubation with anti-Myc (A–C) or anti-VSV-G (D–F) antibodies and then by incubation with Alexa568-conjugated anti-rabbit IgG antibody. Asterisks indicate glomeruli. Scale bar, 50 μm.

Article Snippet: Briefly, cryostat sections (10 μm) of snap-frozen tissues were incubated with anti-HS antibodies RB4CD12 and AO4B08 (1:5) in PBS containing 0.5% blocking reagent at room temperature for 90 min. After washing, the sections were incubated with anti-Myc (1:200; Cell Signaling Technology, Danvers, MA) or anti-VSV-G antibodies (1:200; MBL, Nagoya, Japan) for 60 min.

Techniques: Immunohistochemistry, Incubation, Binding Assay

Journal: The Journal of Biological Chemistry

Article Title: Organ-specific Sulfation Patterns of Heparan Sulfate Generated by Extracellular Sulfatases Sulf1 and Sulf2 in Mice *

doi: 10.1074/jbc.M111.290262

Figure Lengend Snippet: Immunohistochemistry of HS in neonatal lungs. Cryostat sections of the neonatal lungs from wild-type (A and E), Sulf1−/− (B and F), Sulf2−/− (C and G), and Sulf1−/−; Sulf2−/− (D and H) mice were incubated with anti-HS antibodies RB4CD12 (A–D) or AO4B08 (E–H). The antibody binding was detected by incubation with anti-Myc (A–D) or anti-VSV-G (E–H) antibodies and then by incubation with Alexa568-conjugated anti-rabbit IgG antibody. b, bronchus; v, blood vessels. Scale bar, 50 μm.

Article Snippet: Briefly, cryostat sections (10 μm) of snap-frozen tissues were incubated with anti-HS antibodies RB4CD12 and AO4B08 (1:5) in PBS containing 0.5% blocking reagent at room temperature for 90 min. After washing, the sections were incubated with anti-Myc (1:200; Cell Signaling Technology, Danvers, MA) or anti-VSV-G antibodies (1:200; MBL, Nagoya, Japan) for 60 min.

Techniques: Immunohistochemistry, Incubation, Binding Assay